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fu teto gateway  (Addgene inc)


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    Structured Review

    Addgene inc fu teto gateway
    Fu Teto Gateway, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fu+teto+gateway/FU-tetO-Gateway+(Plasmid+%2343914)/pmc12048643-12-0-5
    Average 93 stars, based on 12 article reviews
    fu teto gateway - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Construct:

    Article Title: Mutational profiling of SARS-CoV-2 papain-like protease reveals requirements for function, structure, and drug escape.
    Article Snippet: The FRET biosensor was shuttled into pMX-Gateway-IRES-Hygro (gift from Andrew Brooks, University of Queensland) with LR clonase (Invitrogen, #11791020). .. The PLpro recipient vector, FU-tetO-Gateway-rtTA-2APuro (Supplementary Fig. 2c), was constructed from FU-tetO-Gateway, a gift from John Gearhart (Addgene plasmid #43914; http://n2t.net/ addgene:43914; RRID:Addgene_43914), pMSCV-puro56 and pFTRE3G57. .. Briefly, the PGK driven Puromycin resistance cassette from pMSCVpuro was PCR amplified with oligos containing XmaI sites.

    Article Title: Mutational profiling of SARS-CoV-2 papain-like protease reveals requirements for function, structure, and drug escape
    Article Snippet: The FRET biosensor was shuttled into pMX-Gateway-IRES-Hygro (gift from Andrew Brooks, University of Queensland) with LR clonase (Invitrogen, #11791020). .. The PLpro recipient vector, FU-tetO-Gateway-rtTA-2A-Puro (Supplementary Fig. ), was constructed from FU-tetO-Gateway, a gift from John Gearhart (Addgene plasmid #43914; http://n2t.net/addgene:43914 ; RRID:Addgene_43914), pMSCV-puro and pFTRE3G . .. Briefly, the PGK driven Puromycin resistance cassette from pMSCV-puro was PCR amplified with oligos containing XmaI sites.

    Plasmid Preparation:

    Article Title: Mutational profiling of SARS-CoV-2 papain-like protease reveals requirements for function, structure, and drug escape.
    Article Snippet: The FRET biosensor was shuttled into pMX-Gateway-IRES-Hygro (gift from Andrew Brooks, University of Queensland) with LR clonase (Invitrogen, #11791020). .. The PLpro recipient vector, FU-tetO-Gateway-rtTA-2APuro (Supplementary Fig. 2c), was constructed from FU-tetO-Gateway, a gift from John Gearhart (Addgene plasmid #43914; http://n2t.net/ addgene:43914; RRID:Addgene_43914), pMSCV-puro56 and pFTRE3G57. .. Briefly, the PGK driven Puromycin resistance cassette from pMSCVpuro was PCR amplified with oligos containing XmaI sites.

    Article Title: Mutational profiling of SARS-CoV-2 papain-like protease reveals requirements for function, structure, and drug escape
    Article Snippet: The FRET biosensor was shuttled into pMX-Gateway-IRES-Hygro (gift from Andrew Brooks, University of Queensland) with LR clonase (Invitrogen, #11791020). .. The PLpro recipient vector, FU-tetO-Gateway-rtTA-2A-Puro (Supplementary Fig. ), was constructed from FU-tetO-Gateway, a gift from John Gearhart (Addgene plasmid #43914; http://n2t.net/addgene:43914 ; RRID:Addgene_43914), pMSCV-puro and pFTRE3G . .. Briefly, the PGK driven Puromycin resistance cassette from pMSCV-puro was PCR amplified with oligos containing XmaI sites.

    other:


    Over Expression:

    Article Title: A novel transcription factor combination for direct reprogramming to a spontaneously contracting human cardiomyocyte-like state.
    Article Snippet: All plasmids used in this study have been deposited in Addgene. .. To generate doxycycline-inducible lentiviral plasmids for overexpression of candidate factors, ORF cDNA was inserted into the FU-tetO-Gateway (Addgene 43914) backbone using Gateway cloning. ..

    Cloning:

    Article Title: A novel transcription factor combination for direct reprogramming to a spontaneously contracting human cardiomyocyte-like state.
    Article Snippet: All plasmids used in this study have been deposited in Addgene. .. To generate doxycycline-inducible lentiviral plasmids for overexpression of candidate factors, ORF cDNA was inserted into the FU-tetO-Gateway (Addgene 43914) backbone using Gateway cloning. ..



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